The pressure layer represents biological pressure caused by introduction of non-indigenous species. The data is obtained from core indicator Trend in the arrival of new non-indigenous species (BSEP 129b: http://www.helcom.fi/Lists/Publications/BSEP129B.pdf). For the Baltic Sea Impact Index, the layer was normalized.

Distribution of blue mussel based on data submission by HELCOM contracting parties. Mainly pointwise occurrences of Mytilus spp. were submitted, originally gathered in national mapping and monitoring campaigns, or for scientific research. Point data from Poland was digitized based on Polish Marine Atlas. From Lithuania, a polygon delineating reefs was used to present Mytilus occurrence. For Germany, point data was complemented with a model describing Mytilus biomass in the German marine area (Darr et al. 2014), where predicted biomasses > 1g dw/ m2 were included as presence. From Estonian waters, a predictive model was used (200m resolution), that was converted to presence/absence using minimized difference threshold (MDT) criteria. All data (points, polygon and the raster presenting predicted presence of Mytilus) were generalized to 5km x 5km grid cells.

Input of heat pressure dataset contains delta heat values from warm water discharge of - Discharge of warm water from nuclear power plants - Fossil fuel energy production. The Discharge of warm water from nuclear power plants was requested from HELCOM Contracting Parties. Average temperature change of the cooling water (°C) and amount of cooling water (m3) was used to calculate the heat load in TWh. No data on heat load was available for the Leningrad nuclear power plant; therefore the average heat load (TWh) of discharge of warm water from nuclear power plants was given. No heat load information was available for fossil fuel energy production sites. Heat load of 1 (TWh) was given to all production sites, based on average heat load of individual production site in Helsinki from recent years. 1 km buffer was used for both datasets with steep decline around the outlet. Overlapping areas were summed. Heat load from both layers were summed and the layer was normalized.

Physical loss pressure layer combines all human activities that cause physical loss of seabed. The pressure is given as area lost in each cell (km2). For the polygon datasets the area was assumed to be the lost area. For line and point datasets spatial extents were calculated with buffers (below in brackets). If no buffer extent is indicated, the data was reported as polygon. The human activities used for the physical loss pressure: - Bridges (2 m) - Cables (operational; 1,5 m) - Coastal defence and flood protection (area of polygon, 50 m for lines) - Dredging (capital dredging, Area of polygon or a 25/50 m buffer for <5000 m3 / >5000m3 points) - Extraction of sand and gravel - Finfish mariculture (150 m) - Harbours (polygon with 200 m buffer) - Land claim (area of polygon, 30m buffer for lines) - Marinas and leisure harbours (200 m) - Oil platforms (25 m) - Oil terminals and refineries (200 m) - Pipelines (operational; 15 m) - Shellfish mariculture (area of polygon, 150 m points) - Watercourse modification (50 m) - Wind turbines (operational; 30m point location of turbine) The datasets were first processed separately covering the whole Baltic Sea and then merged into one uniform data layer and minimizing the effect of overlapping areas. Polygon areas were clipped with coastline to remove buffered areas that reached to land.

This dataset is built from the following Human activities dataset: • Hunting of seals The number of hunted seals (see separate metadata on hunting of seals) were averaged over 2011-2014 separately for grey seals, ringed seals and harbour seals (e.g. number of hunted grey seals / year). In Sweden the numbers of hunted grey seals in 2011 (74) were reported for the whole Swedish territorial waters), but here the numbers were set only to Swedish Gulf of Bothnia, as corresponding numbers were reported there in 2013 (75) and 2014 (65). The area of the reporting unit was used to calculate the number of hunted seals / km2 and the data was converted to 1km x 1km grid. For the Baltic Sea Impact Index, the values were normalized. Normalized value 0.5 was set to the level of quota for hunting of seal species in the Baltic Sea. The following quotas for hunting were used: Grey seal: 2000, Ringed seal: 350, Harbour seal 230.

Potential cumulative impacts of eutrophication and hazardous substances assesses the cumulative potential effect of eutrophication and hazardous substances over all ecosystem components. The evaluation is based on the pressure layer on eutrophication and hazardous substances, combined with information on all ecosystem components (57 layers) included in SPIA for HOLAS 3. The thematic analyses is calculated for each assessment unit (1 km2 grid cells) and the data set covers the time period 2016-2021. Spatial Pressure and Impact Assessment (SPIA) is the framework for assessing spatial and cumulative pressures and impacts in HOLAS 3, and this analyses present a thematic assessment including only a certain subset of layers. The framework also includes results for the Baltic Sea Impact Index (full cumulative impact assessment), Baltic Sea Pressure Index (full cumulative pressure assessment), and other thematic assessments where a subset of pressure and ecosystem layers are used. For more info please - visit the HOLAS 3 website (http://stateofthebalticsea.helcom.fi/) - download the report thematic assessment of spatial distribution of pressures and impacts 2016-2021 (https://helcom.fi/post_type_publ/holas3_spa) - or check out the HELCOM SPIA online tool to make calculations for any desired combination of pressures and ecosystem layers (https://maps.helcom.fi/website/bsii/). Please scroll down to "Lineage" for a more detailed description of the methodology.

Broad-scale habitat maps for the Baltic Sea have been produced in the EUSeaMap project in 2016. For German and Estonian marine areas, national (more accurate) datasets were used. German data included both substrate and light information (division into infralittoral/circalittoral). Estonian data included only substrate and the division into light regimes was obtained from the EuSeaMap data. Here, the habitat class “circalittoral mixed substrate” includes classes “mixed sediment” of the original data, in the circalittoral zone. The original polygon maps have been converted to 1 km x 1 km grid. The scale of the substrate data used in broad-scale habitat maps varies from 1:250 000 to 1:1M (data from EMODnet Geology). Coarser resolution data has been used in areas, where 1: 250 000 substrate data has not been available. Due to different scales used, the habitat classes may show different sized patterns in different areas.

Distribution of eelgrass based on data submission by HELCOM contracting parties. Mainly pointwise occurrences of eelgrass were submitted, originally gathered in national mapping and monitoring campaigns, or for scientific research. Polygon data from Puck Bay (Poland) was digitized based on Polish Marine Atlas and Orlowo cliff area was added based on expert knowledge. From Estonian waters, a predictive model was used (200m resolution), that was converted to presence/absence using minimized difference threshold (MDT) criteria. All data (points, polygon and the raster presenting predicted presence of eelgrass in the Estonian waters) were generalized to 5km x 5km grid cells.

This map shows the distribution and abundance of harbour seals across the Baltic Sea. The map was originally created for HELCOM Red list assessment of the Baltic Sea, using seal expert consultation. For the Baltic Sea Impact Index, the map was modified to represent four abundance classes, based on expert consultation. The map has been updated from the 1st version of HOLASII, based on expert consultation (HELCOM Seal EG).

This dataset is built from following Human activities datasets: • http://metadata.helcom.fi/geonetwork/srv/eng/catalog.search#/metadata/81c917ea-492d-48e2-9f00-e1bb7fe3e4fc • http://metadata.helcom.fi/geonetwork/srv/eng/catalog.search#/metadata/4fcd51dd-b8be-4e83-8cad-37c566782e8f The game hunting of seabirds data (see separate metadata): The total number of hunted seabirds were averaged over 2011-2015 (number of hunted seabirds / year). The area of the reporting unit was used to calculate the number of hunted seabirds / km2 and the data was converted to 1km x 1km grid. The predator control of seabirds data (see separate metadata): The total number of hunted cormorants were averaged over 2011-2015 (number of hunted cormorants / year). The area of the reporting unit was used to calculate the number of hunted cormorants / km2 and the data was converted to 1km x 1km grid. The two datasets were first separately log transformed and then summed, to get the total value for each grid cell. Zero values were given to all grid cells with no reported seabird hunting activity. The layer was normalized.